研究業績 Publications

Selected papers | 2025 | 2024 | 2023 | 2022 | 2021 | 2020 | 2019 | 2018 | 2017 | 2016 | 2015 | 2014 | 2013 | 2012 | 2011 | 2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | ~2002

2025

• Kawanabe-Kobayashi R, Uchiyama S, Yoshihara K, Kojima D, McHugh T, Hatada I, Matsui K, Tanaka KF, Tsuda M
  Descending locus coeruleus noradrenergic signaling to spinal astrocyte subset is required for stress-induced pain facilitation
  eLife, 2025

• Kohno K, Shirasaka R, Hirose K, Masuda T, Tsuda M
  Interleukin-4 induces CD11c+ microglia leading to amelioration of neuropathic pain in mice
  eLife, 2025

• Sueto D, I E, Onishi A, Tsuda M
  Spinal dorsal horn neurons involved in the alleviating effects of cannabinoid receptor agonists on neuropathic allodynia-like behaviors in rats
  J Pharmacol Sci 157(4): 253-260 (2025)

• Nakamura H, Endo T, Tsuda M
  Pharmacological Profile of NCP-322, a Novel G Protein-Coupled Receptor 119 Agonist, as an Orally Active Therapeutic Agent for Type 2 Diabetes Mellitus.
  Biol Pharm Bull 48(1): 65-74 (2025)

• Fuma K, Iitani Y, Imai K, Ushida T, Tano S, Yoshida K, Yokoi A, Miki R, Kidokoro H, Sato Y, Hara Y, Ogi T, Nomaki K, Tsuda M, Komine O, Yamanaka K, Kajiyama H, Kotani T
  Prenatal inflammation impairs early CD11c-positive microglia induction and delays myelination in neurodevelopmental disorders.
  Commun Biol 8(1): 75 (2025)

2024

• Fujii K, Miyagawa R, Tanaka R, Saito M, Tanaka S, Shiratori-Hayashi M, Tsuda M, Dong X, Fujii M.
  MrgprA3+ primary sensory neurons mediate acute allergic itch responses in atopic dermatitis model mice
  Biol Pharm Bull 47(10): 1624-1630 (2024)

• Shigetomi E, Suzuki H, Hirayama YJ, Sano F, Nagai Y, Yoshihara K, Koga K, Tateoka T, Yoshioka H, Shinozaki Y, Kinouchi H, Tanaka KF, Bito H, Tsuda M, Koizumi S
  Disease-relevant upregulation of P2Y1 receptor in astrocytes enhances neuronal excitability via IGFBP2
  Nat Commun 15(1): 6525 (2024)

• Yamasaki A, Imanishi I, Tanaka K, Ohkawa Y, Tsuda M, Masuda T
  IRF8 and MAFB drive distinct transcriptional machineries in different resident macrophages of the central nervous system
  Commun Biol 7(1): 896 (2024)

• Iwasaki R, Kohro Y, Tsuda M
  A method for selective and efficient isolation of gray matter astrocytes from the spinal cord of adult mice
  Mol Brain 17(1): 25 (2024)

• Nomaki K, Fujikawa R, Masuda T, Tsuda M
  Spatiotemporal dynamics of the CD11c+ microglial population in the mouse brain and spinal cord from developmental to adult stages
  Mol Brain 17(1): 24 (2024)

• Sueto D, Ishibashi I, Eriko I, Tashima R, Yoshikawa Y, Yamaura K, Tsuda M
  Interaction between dorsal horn neuron subsets operated by a neuropeptide Y and prodynorphin promoter and its contribution to Aβ fiber-induced allodynia-like behavior in rats
  Pain Res 39(1): 40-45 (2024)

• Koga K, Kobayashi K, Tsuda M, Pickering AE, Furue H
  Anterior cingulate cross-hemispheric inhibition via the claustrum resolves painful sensory conflict
  Commun Biol 7(1): 330 (2024)

• Sueto D, Onishi A, I E, Yoshikawa Y, Tsuda M
  Laminar-selective spinal astrocyte population capable of converting tactile information into nociceptive in rats
  J Pharmacol Sci 154(4): 312-315 (2024)

2023

• Ono G, Kobayakawa K, Saiwai H, Tamaru T, Iura H, Haruta Y, Kitade K, Iida K, Kawaguchi K, Matsumoto Y, Tsuda M, Tamura T, Ozato K, Inoue K, Konno DJ, Maeda T, Okada S, Nakashima Y
  Macrophages play a leading role in determining the direction of astrocytic migration in spinal cord injury via ADP-P2Y1R axis.
  Sci Rep 13: 11177 (2023)

• Tsuda M, Masuda T, Kohno K
  Microglial diversity in neuropathic pain.
  Trends Neurosci 46: 597-610(2023) 　★Selected Paper

• Fujikawa R, Tsuda M
  The Functions and Phenotypes of Microglia in Alzheimer’s Disease.
  Cells 12: 1207 (2023)

• Ono T, Yamashita T, Kano R, Inoue M, Okada S, Kano K, Koizumi S, Iwabuchi K, Hirabayashi Y, Matsuo I, Nakashima Y, Kamiguchi H, Kohro Y, Tsuda M
  GPR55 contributes to neutrophil recruitment and mechanical pain induction after spinal cord compression in mice.
  Brain Behav Immun 110:276-287(2023)

• Koga K, Kobayashi K, Tsuda M, Kubota K, Kitano Y, Furue M
  Voltage-gated calcium channel subunit α2δ-1 in spinal dorsal horn neurons contributes to aberrant excitatory synaptic transmission and mechanical hypersensitivity after peripheral nerve injury.
  Front Mol Neurosci 16: 1099925 (2023)

2022

• Ishibashi T, Sueto D, Yoshikawa Y, Koga K, Yamaura K, Tsuda M
  Identification of spinal inhibitory interneurons required for attenuating effect of duloxetine on neuropathic allodynia-like signs in rats.
  Cells 11(24): 4051 (2022)

• Shiratori-Hayashi M, Tsuda M
  IP3R1-dependent astrocyte calcium signaling in chronic itch.
  Neurosci Res S0168-0102(22)00258-9 (2022)

• Takeda I, Yoshihara K, Cheung DL, Kobayashi T, Agetsuma M, Tsuda M, Eto K, Koizumi S, Wake H, Moorhouse AJ, Nabekura J
  Controlled activation of cortical astrocytes modulates neuropathic pain-like behaviour.
  Nat Commun 13(1): 4100 (2022)

• Ishibashi T, Yoshikawa Y*, SuetoD*, Tashima R, Tozaki-Saitoh H, Koga K, Yamaura K, Tsuda M (*equally contributor)
  Selective involvement of a subset of spinal dorsal horn neurons operated by a prodynorphin promoter in Aβ fiber-mediated neuropathic allodynia-like behavioral responses in rats.
  Front Mol Neurosci 15: 911122 (2022)

• Fujimori K, Sekine M, Watanabe M, Tashima R, Tozaki-Saitoh H, Tsuda M
  Chemogenetic silencing of spinal cord-projecting cortical neurons attenuates Aβ fiber-derived neuropathic allodynia in mice.
  Neurosci Res181: 115-119 (2022)

• Kanehisa K, Koga K, Maejima S, Shiraishi Y, Asai K, Shiratori-Hayashi M, Xiao MF, Sakamoto H, Worley PF, Tsuda M
  Neuronal pentraxin 2 is required for facilitating excitatory synaptic inputs onto spinal neurons involved in pruriceptive transmission in a model of chronic itch.
  Nat Commun 13(1): 2367 (2022) 　★Selected Paper

• Masuda T, Amann L, Monaco G, Sankowski R, Staszewski O, Krueger M, Del Gaudio F, He L, Paterson N, Nent E, Fernandez-Klett F, Yamasaki A, Frosch M, Fliegauf M, Bosch LFP, Ulupinar H, Hagemeyer N, Schreiner D, Dorrier C, Tsuda M, Grothe C, Joutel A, Daneman R, Betsholtz C, Lendahl U, Knobeloch KP, Lammermann T, Priller J, Kierdorf K, Prinz M
  Specification of CNS macrophage subsets occurs postnatally in defined niches.
  Nature 604(7907): 740-748 (2022) 　★Selected Paper

• Kohno K, Shirasaka R, Yoshihara K, Mikuriya S, Tanaka K, Takanami K, Inoue K, Sakamoto H, Ohkawa Y, Masuda T, Tsuda M
  A spinal microglia population involved in remitting and relapsing neuropathic pain.
  Science 376(6588): 86-90 (2022) 　★Selected Paper

• Shinozaki C, Kohno K, Shiroishi M, Takahashi D, Yoshikawa Y, Abe Y, Hamase K, Nakakido M, Tsumoto K, Inoue K, Tsuda M, Ueda T
  Improvement of the affinity of an anti-rat P2X4 receptor antibody by introducing electrostatic interactions.
  Sci Rep 12(1): 131 (2022)

• Uchiyama S, Yoshihara K, Kawanabe R, Hatada I, Koga K, Tsuda M
  Stress-induced antinociception to noxious heat requires α1A-adrenaline receptors of spinal inhibitory neurons in mice.
  Mol Brain 15(1): 6 (2022)

2021

• Shiratori-Hayashi M, Tsuda M
  Spinal glial cells in itch modulation.
  Pharmacol Res Perspect 9(6): e00754 (2021)

• Kawanabe R, Yoshihara K, Hatada I, Tsuda M
  Activation of spinal dorsal horn astrocytes by noxious stimuli involves descending noradrenergic signaling.
  Mol Brain 14(1): 79 (2021)

• Kohno K, Tsuda M
  Role of microglia and P2X4 receptors in chronic pain.
  Pain Rep 6(1): e864 (2021)

• Igawa T, Kishikawa S, Abe Y, Tsuda M, Inoue K, Ueda T
  Analysis of binding residues in monoclonal antibody with high affinity for the head domain of the rat P2X4 receptor.
  J Biochem 169(4): 491-496 (2021)

• Inoue K, Tsuda M
  Nociceptive signaling mediated by P2X3, P2X4 and P2X7 receptors.
  Biochem Pharmacol 187: 114309 (2021)

• Shiratori-Hayashi M, Yamaguchi C, Eguchi K, Kohno K, Shiraishi Y, Mikoshiba K, Inoue K, Nishida M, Tsuda M
  Astrocytic STAT3 activation and chronic itch require IP3R1/TRPC-dependent Ca2+ signals in mice.
  J Allergy Clin Immunol 147(4): 1341-1353 (2021) 　★Selected Paper

• Shiraishi Y, Koga K, Yamagata R, Hatada I, Shiratori-Hayashi M, Tsuda M
  α1A-adrenaline receptors in dorsal horn inhibitory neurons have an inhibitory role in the regulation of chloroquine-induced itch in mice.
  Mol Brain 14(1): 55 (2021)

• Yamashita T, Kamikaseda S, Tanaka A, Tozaki-Saitoh H, Caaveiro JMM, Inoue K, Tsuda M
  New inhibitory effects of cilnidipine on microglial P2X7 receptors and IL-1β release: an involvement in its alleviating effect on neuropathic pain.
  Cells 10(2): 434 (2021)

• Tashima R, Koga K, Yoshikawa Y, Sekine M, Watanabe M, Tozaki-Saitoh H, Furue H, Yasaka T, Tsuda M
  A subset of spinal dorsal horn interneurons crucial for gating touch-evoked pain-like behavior.
  Proc Natl Acad Sci USA 118(3): e2021220118 (2021) 　★Selected Paper

• Uchida S, Soya S, Saito YC, Hirano A, Koga K, Tsuda M, Abe M, Sakimura K, Sakurai T
  A discrete glycinergic neuronal population in the ventromedial medulla that induces muscle atonia during REM sleep and cataplexy in mice.
  J Neurosci 41(7): 1582-1596 (2021)

2020

• Koga K, Shiraishi Y, Yamagata R, Tozaki-Saitoh H, Shiratori-Hayashi M, Tsuda M
  Intrinsic braking role of descending locus coeruleus noradrenergic neurons in acute and chronic itch in mice.
  Mol Brain 13(1): 144 (2020)

• Kohro Y, Matsuda T, Yoshihara K, Kohno K, Koga K, Katsuragi R, Oka T, Tashima R, Muneta S, Yamane T, Okada S, Momokino K, Furusho A, Hamase K, Oti T, Sakamoto H, Hayashida K, Kobayashi R, Horii T, Hatada I, Tozaki-Saitoh H, Mikoshiba K, Taylor V, Inoue K, Tsuda M
  Spinal astrocytes in superficial laminae gate brainstem descending control of mechanosensory hypersensitivity.
  Nat Neurosci 23: 1376-1387 (2020)　★Selected Paper

• Konishi H, Okamoto T, Hara Y, Komine O, Tamada H, Maeda M, Osako F, Kobayashi M, Nishiyama A, Kataoka Y, Takai T, Udagawa N, Jung S, Ozato K, Tamura T, Tsuda M, Yamanaka K, Ogi T, Sato K, Kiyama H
  Astrocytic phagocytosis is a compensatory mechanism for microglial dysfunction.
  EMBO J 39(22): e104464 (2020)

• Shiratori-Hayashi M, Tsuda M
  Role of reactive astrocytes in the spinal dorsal horn under chronic itch conditions.
  J Pharmacol Sci 144(3): 147-150 (2020)

• Ono T, Kohro Y, Kohno K, Tozaki-Saitoh H, Nakashima Y, Tsuda M
  Mechanical pain of the lower extremity after compression of the upper spinal cord involves signal transducer and activator of transcription 3-dependent reactive astrocytes and interleukin-6.
  Brain Behav Immun 89: 389-399 (2020)

• Nakagawa T, Yasaka T, Nakashima N, Takeya M, Oshita K, Tsuda M, Yamaura K, Takano M
  Expression of the pacemaker channel HCN4 in excitatory interneurons in the dorsal horn of the murine spinal cord.
  Mol Brain 13(1): 127 (2020)

• Shimoda K, Nishimura A, Sunggip C, Ito T, Nishiyama K, Kato Y, Tanaka T, Tozaki-Saitoh H, Tsuda M, Nishida M
  Modulation of P2Y6R expression exacerbates pressure overload-induced cardiac remodeling in mice.
  Sci Rep 10(1): 13926 (2020)

• Teratani T, Mikami Y, Nakamoto N, Suzuki T, Harada Y, Okabayashi K, Hagihara Y, Taniki N, Kohno K, Sibata S, Miyamoto K, Ishigame H, Chu PS, Sujino T, Suda W, Hattori M, Matsui M, Okada T, Okano H, Inoue M, Yada T, Kitagawa Y, Yoshimura A, Tanida M, Tsuda M, Iwasaki Y, Kanai T
  The liver-brain-gut neural arc maintains the Treg cell niche in the gut.
  Nature 585(7826): 591-596 (2020)

• Ohmichi Y, Ohmichi M, Tashima R, Osuka K, Fukushige K, Kanikowska D, Fukazawa Y, Yawo H, Tsuda M, Naito M, Nakano T
  Physical disuse contributes to widespread chronic mechanical hyperalgesia, tactile allodynia, and cold allodynia through neurogenic inflammation and spino-parabrachio-amygdaloid pathway activation.
  Pain 161(8): 1808-1823 (2020)

• Tozaki-Saitoh H, Sasaki I, Yamashita T, Hosoi M, Kato T, Tsuda M
  Involvement of exchange protein directly activated by cAMP and tumor progression locus 2 in IL-1β production in microglial cells following activation of β-adrenergic receptors.
  J Pharmacol Sci 143(3): 133-140 (2020)

• Koga K*, Yamagata R*, Kohno K, Yamane T, Shiratori-Hayashi M, Kohro Y, Tozaki-Saitoh H, Tsuda M (*equal contributors)
  Sensitization of spinal itch transmission neurons in a mouse model of chronic itch requires an astrocytic factor.
  J Allergy Clin Immunol 145(1): 183-191.e10 (2020)　★Selected Paper

2019

• Tsuda M
  Microglia-mediated regulation of neuropathic pain: molecular and cellular mechanisms.
  Biol Pharm Bull 42(12): 1959-1968 (2019)

• Tozaki-Saitoh H, Tsuda M
  Microglia-neuron interactions in the models of neuropathic pain.
  Biochem Pharmacol 169: 113614 (2019)

• Yamamoto S*, Yamashita T*, Ito M, Caaveiro JMM, Egashira N, Tozaki-Saitoh H, Tsuda M (*equal contributors)
  New pharmacological effect of fulvestrant to prevent oxaliplatin-induced neurodegeneration and mechanical allodynia in rats.
  Int J Cancer 145(8): 2107-2113 (2019)

• Kobayakawa K, Ohkawa Y, Yoshizaki S, Tamaru T, Saito T, Kijima K, Yokota K, Hara M, Kubota K, Matsumoto Y, Harimaya K, Ozato K, Masuda T, Tsuda M, Tamura T, Inoue K, Edgerton VR, Iwamoto Y, Nakashima Y, Okada S
  Macrophage centripetal migration drives spontaneous healing process after spinal cord injury.
  Sci Adv 5(5): eaav5086 (2019)

• Tsuda M
  New approach for investigating neuropathic allodynia by optogenetics.
  Pain 160 (Suppl 1): S53-S58 (2019)

• Yasui M, Menjyo Y, Tokizane K, Shiozawa A, Tsuda M, Inoue K, Kiyama H
  Hyperactivation of proprioceptors induces microglia-mediated long-lasting pain in a rat model of chronic fatigue syndrome.
  J Neuroinflammation 16(1): 67 (2019)

• Igawa T, Kishikawa S, Abe Y, Yamashita T, Nagai S, Shiroishi M, Shinozaki C, Tanaka H, Tozaki-Saitoh H, Tsuda M, Inoue K, Ueda T
  Evidence for detection of rat P2X4 receptor expressed on cells by generating monoclonal antibodies recognizing the native structure.
  Purinergic Signal 15(1): 27-35 (2019)

• Tozaki-Saitoh H, Masuda J, Kawada R, Kojima C, Yoneda S, Masuda T, Inoue K, Tsuda M
  Transcription factor MafB contributes to the activation of spinal microglia underlying neuropathic pain development.
  Glia 67(4): 729-740 (2019)

• Shiratori-Hayashi M, Hasegawa A, Toyonaga H, Andoh T, Nakahara T, Kido-Nakahara M, Furue M, Kuraishi Y, Inoue K, Dong X, Tsuda M
  Role of P2X3 receptors in scratching behavior in mouse models.
  J Allergy Clin Immunol 143(3): 1252-1254 (2019)　★Selected Paper

2018

• Sasaguri T, Taguchi T, Murata Y, Kobayashi K, Iizasa S, Iizasa E, Tsuda M, Hirakawa N, Hara H, Yoshida H, Yasaka T
  Interleukin-27 controls basal pain threshold in physiological and pathological conditions.
  Sci Rep 8: 11022 (2018)　

• Chen T, Taniguchi W, Chen QY, Tozaki-Saitoh H, Song Q, Liu RH, Koga K, Matsuda T, Kaito-Sugimura Y, Wang J, Li ZH, Lu YC, Inoue K, Tsuda M, Li YQ, Nakatsuka T, Zhuo M
  Top-down descending facilitation of spinal sensory excitatory transmission from the anterior cingulate cortex.
  Nat Commun 9: 1886 (2018)　

• Kohno K*, Kitano J*, Kohro Y, Tozaki-Saitoh H, Inoue K, Tsuda M (*equal contributors)
  Temporal kinetics of microgliosis in the spinal dorsal horn after peripheral nerve injury in rodents.
  Biol Pharm Bull 41: 1096-1102 (2018)　

• Yoshihara K*, Matsuda T*, Kohro Y, Tozaki-Saitoh H, Inoue K, Tsuda M (*equal contributors)
  Astrocytic Ca2+ responses in the spinal dorsal horn by noxious stimuli to the skin.
  J Pharmacol Sci 137(1):101-104 (2018)　

• Inoue K, Tsuda M
  Microglia in neuropathic pain: cellular and molecular mechanisms and therapeutic potential.
  Nat Rev Neurosci 19: 138-152 (2018)　★Selected Paper　

• Tashima R, Koga K, Sekine M, Kanehisa K, Kohro Y, Tominaga K, Matsushita K, Tozaki-Saitoh H, Fukazawa Y, Inoue K, Yawo H, Furue H, Tsuda M
  Optogenetic activation of non-nociceptive Aβ fibers induces neuropathic pain-like sensory and emotional behaviors after nerve injury in rats.
  eNeuro 5(1). pii: ENEURO.0450-17.2018 (2018)　★Selected Paper　

• Tsuda M
  Modulation of pain and itch by spinal glia.
  Neurosci Bull 34: 178-185 (2018)　

• Tsuda M
  Astrocytes in the spinal dorsal horn and chronic itch.
  Neurosci Res 126: 9-14 (2018)　

• Yamamoto S, Egashira N, Tsuda M, Masuda S
  Riluzole prevents oxaliplatin-induced cold allodynia via inhibition of overexpression of transient receptor potential melastatin 8 in rats.
  J Pharmacol Sci 138(3): 214-217 (2018)　

2017

• Ohgidani M, Kato TA, Hosoi M, Tsuda M, Hayakawa K, Hayaki C, Iwaki R, Sagata N, Hashimoto R, Inoue K, Sudo N, Kanba S
  Fibromyalgia and microglial TNF-α: Translational research using human blood induced microglia-like cells.
  Sci Rep 7(1):11882 (2017)　

• Kanehisa K, Shiratori-Hayashi M, Koga K, Tozaki-Saitoh H, Kohro Y, Takamori K, Tsuda M
  Specific activation of inhibitory interneurons in the spinal dorsal horn suppresses repetitive scratching in mouse models of chronic itch.
  J Dermatol Sci 88: 251-254 (2017)　

• Nishimura A, Sunggip C, Oda S, Numaga-Tomita T, Tsuda M, Nishida M
  Purinergic P2Y receptors: Molecular diversity and implications for treatment of cardiovascular diseases.
  Pharmacol Ther 180: 113-128 (2017)　

• Koga K, Kanehisa K, Kohro Y, Shiratori-Hayashi M, Tozaki-Saitoh H, Inoue K, Furue H, Tsuda M
  Chemogenetic silencing of GABAergic dorsal horn interneurons induces morphine-resistant spontaneous nocifensive behaviours.
  Sci Rep 7(1): 4739 (2017)　

• Sunggip C, Nishimura A, Shimoda K, Numaga-Tomita T, Tsuda M, Nishida M
  Purinergic P2Y6 receptors: A new therapeutic target of age-dependent hypertension.
  Pharmacol Res 120: 51-59 (2017)　

• Tsuda M
  P2 receptors, microglial cytokines and chemokines, and neuropathic pain.
  J Neurosci Res 95: 1319-1329 (2017)　

• Tsuda M, Koga K, Chen T, Zhuo M
  Neuronal and microglial mechanisms for neuropathic pain in the spinal dorsal horn and anterior cingulate cortex.
  J Neurochem 141: 486-498 (2017)　

• Tozaki-Saitoh H, Miyata H, Yamashita T, Matsushita K, Tsuda M, Inoue K
  P2Y12 receptors in primary microglia activate nuclear factor of activated T cell signaling to induce C-C chemokine 3 expression.
  J Neurochem 141: 100-110 (2017)　

• Tsuda M
  Spinal dorsal horn astrocytes: new players in chronic itch.
  Allergol Int 66: 31-35 (2017)　

• Nakaya M, Watari K, Tajima M, Nakaya T, Matsuda S, Ohara H, Nishihara H, Yamaguchi H, Hashimoto A, Nishida M, Nagasaka A, Horii Y, Ono H, Iribe G, Inoue R, Tsuda M, Inoue K, Tanaka A, Kuroda M, Nagata S, Kurose H
  Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction.
  J Clin Invest 127: 383-401 (2017) 　

2016

• Yamashita T, Yamamoto S, Zhang J, Kometani M, Tomiyama D, Kohno K, Tozaki-Saitoh H, Inoue K, Tsuda M
  Duloxetine inhibits microglial P2X4 receptor function and alleviates neuropathic pain after peripheral nerve injury.
  PLoS One 11: e0165189 (2016)　

• Koyanagi S, Kusunose N, Taniguchi M, Akamine T, Kanado Y, Ozono Y, Masuda T, Kohro Y, Matsunaga N, Tsuda M, Salter MW, Inoue K, Ohdo S
  Glucocorticoid regulation of ATP release from spinal astrocytes underlies diurnal exacerbation of neuropathic mechanical allodynia.
  Nat Commun 7: 13102 (2016)　

• Matsunaga N, Ikeda E, Kakimoto K, Watanabe M, Shindo N, Tsuruta A, Ikeyama H, Hamamura K, Higashi K, Yamashita T, Kondo H, Yoshida Y, Matsuda M, Ogino T, Tokushige K, Itcho K, Furuichi Y, Nakao T, Yasuda K, Doi A, Amamoto T, Aramaki H, Tsuda M, Inoue K, Ojida A, Koyanagi S, Ohdo S
  Inhibition of G0/G1 Switch 2 Ameliorates Renal Inflammation in Chronic Kidney Disease.
  EBioMedicine S2352-3964: 30463-30467 (2016)　

• Matsumura Y*, Yamashita T*, Sasaki A*, Nakata E, Kohno K, Masuda T, Tozaki-Saitoh H, Imai T, Kuraishi Y, Tsuda M#, Inoue K (*equal contributors, #corresponding author)
  A novel P2X4 receptor-selective antagonist produces anti-allodynic effect in a mouse model of herpetic pain.
  Sci Rep 6: 324617 (2016)　

• Masuda T, Ozono Y*, Mikuriya S*, Kohro Y, Tozaki-Saitoh H, Iwatsuki K, Uneyama H, Ichikawa R, Salter MW, Tsuda M#, Inoue K#? (*equal contributors, #corresponding authors)
  Dorsal horn neurons release extracellular ATP in a VNUT-dependent manner that underlies neuropathic pain.
  Nat Commun 7: 12529 (2016)　★Selected Paper

• Tsuda M, Inoue K
  Neuron-microglia interaction by purinergic signaling in neuropathic pain following neurodegeneration.
  Neuropharmacology 104: 76-81 (2016)　

• Masuda T, Tsuda M, Inoue K
  Transcriptional regulation in microglia and neuropathic pain.
  Pain Manag 6: 91-94 (2016)　

• Tashima R, Mikuriya S, Tomiyama D, Shiratori-Hayashi M, Yamashita T, Kohro Y, Tozaki-Saitoh H, Inoue K, Tsuda M
  Bone marrow-derived cells in the population of spinal microglia after peripheral nerve injury.
  Sci Rep 6: 23701 (2016)　

• Nishimura A, Sunggip C, Tozaki-Saitoh H, Shimauchi T, Numaga-Tomita T, Hirano K, Ide T, Boeynaems JM, Kurose H, Tsuda M, Robaye B, Inoue K, Nishida M.
  Purinergic P2Y6 receptors heterodimerize with angiotensin AT1 receptors to promote angiotensin II-induced hypertension.
  Sci Signal 9: ra7 (2016)　

2015

• Kohro Y, Sakaguchi E, Tashima R, Tozaki-Saitoh H, Okano H, Inoue K, Tsuda M
  A new minimally-invasive method for microinjection into the mouse spinal dorsal horn.
  Sci Rep 5: 14306 (2015)　★Selected Paper
• Tsuda M, Inoue K
  Neuron-microglia interaction by purinergic signaling in neuropathic pain following neurodegeneration.
  Neuropharmacology (2015)
• Masuda T, Iwamoto S, Mikuriya S, Tozaki-Saitoh H, Tamura T, Tsuda M, Inoue K
  Transcription factor IRF1 is responsible for IRF8-mediated IL-1β expression in reactive microglia.
  J Pharmacol Sci 128: 216-220 (2015)
• Shiratori-Hayashi M, Koga K, Tozaki-Saitoh H, Kohro Y, Toyonaga H, Yamaguchi C, Hasegawa A, Nakahara T, Hachisuka J, Akira S, Okano H, Furue M, Inoue K, Tsuda M
  STAT3-dependent reactive astrogliosis in the spinal dorsal horn underlies chronic itch.
  Nat Med 21: 927-931 (2015) 　★Selected Paper

2014

• Akagi T, Matsumura Y, Yasui M, Minami E, Inoue H, Masuda T, Tozaki-Saitoh H, Tamura T, Mizumura K, Tsuda M, Kiyama H, Inoue K
  Interferon regulatory factor 8 expressed in microglia contributes to tactile allodynia induced by repeated cold stress in rodents.
  J Pharmacol Sci 126: 172-176 (2014)
• Ochi-Ishi R, Nagata K, Inoue T, Tozaki-Saitoh H, Tsuda M, Inoue K
  Involvement of the chemokine CCL3 and the purinoceptor P2X7 in the spinal cord in paclitaxel-induced mechanical allodynia.
  Mol Pain 10: 53 (2014)
• Hiroshi Shimoyama, Makoto Tsuda, Takahiro Masuda, Ryohei Yoshinaga, Keiko Tsukamoto, Hidetoshi Tozaki-Saitoh, Kazuhide Inoue
  Spinal cord is the primary site of action of the cannabinoid CB2 receptor agonist JWH133 that suppresses neuropathic pain: possible involvement of microglia.
  Open Pain J 7: 1-8 (2014)
• Yasui M, Yoshimura T, Takeuchi S, Tokizane K, Tsuda M, Inoue K, Kiyama H
  A Chronic fatigue syndrome model demonstrates mechanical allodynia and muscular hyperalgesia via spinal microglial activation.
  Glia 62: 1407-1417 (2014)
• Masuda T, Iwamoto S, Yoshinaga R, Tozaki-Saitoh H, Nishiyama A, Mak TW, Tamura T, Tsuda M#, Inoue K# (# Corresponding authors)
  Transcription factor IRF5 drives P2X4R⁺-reactive microglia gating neuropathic pain.
  Nat Commun 5: 3771 (2014)　★Selected Paper
• Masuda T, Nishimoto N, Tomiyama D, Matsuda T, Tozaki-Saitoh H, Tamura T, Kohsaka S, Tsuda M, Inoue K
  IRF8 is a transcriptional determinant for microglial motility.
  Purinergic Signal 10: 515-521 (2014)
• Matsushita K*, Tozaki-Saitoh H*, Kojima C, Masuda T, Tsuda M, Inoue K, Hoka S (* Equal contributors)
  Chemokine (C-C motif) receptor 5 is an important pathological regulator in the development and maintenance of neuropathic pain.
  Anesthesiology 120: 1491-503 (2014)

2013

• Tsuda M, Masuda T, Tozaki-Saitoh H, Inoue K
  P2X4 receptors and neuropathic pain.
  Front Cell Neurosci 7: 191 (2013)
• Masuda T, Tsuda M, Tozaki-Saitoh H, Inoue K
  Intrathecal infusion of microglia cells.
  Methods Mol Biol 1041: 291-294 (2013)
• Masuda T, Tsuda M, Tozaki-Saitoh H, Inoue K
  Lentiviral transduction of cultured microglia.
  Methods Mol Biol 1041: 63-67 (2013)
• Nakaya M, Tajima M, Kosako H, Nakaya T, Hashimoto A, Watari K, Nishihara H, Ohba M, Komiya S, Tani N, Nishida M, Taniguchi H, Sato Y, Matsumoto M, Tsuda M, Kuroda M, Inoue K, Kurose H
  GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance.
  Nat Commun 4: 1532 (2013)
• Tsuda M#, Masuda T, Tozaki-Saitoh H, Inoue K
  Microglial regulation of neuropathic pain.
  J Pharmacol Sci 121: 89-94 (2013)
• Igawa T, Higashi S, Abe Y, Ohkuri T, Tanaka H, Morimoto S, Yamashita T, Tsuda M, Inoue K, Ueda T
  Preparation and characterization of a monoclonal antibody against the refolded and functional extracellular domain of rat P2X4 receptor.
  J Biochem 153(3): 275-282 (2013)

2012

• Muratake H, Ito A, Toda T, Suzuki H, Fukasawa H, Tsuda M, Inoue K, Sugiyama K, Shudo K
  (R)- and (S)-4-Amino-3-(trimethylsilyl)methylbutanoic acids ameliorate neuropathic pain without central nervous system-related side effects.
  Bioorg Med Chem Lett 22(24): 7602-7604 (2012)
• Inoue K, Tsuda M
  P2X4 receptors of microglia in neuropathic pain.
  CNS Neurol Disord Drug Targets 11: 699-704 (2012)
• Sun L, Wu Z, Hayashi Y, Peters C, Tsuda M, Inoue K, Nakanishi H
  Microglial Cathepsin B contributes to the initiation of peripheral inflammation-induced chronic pain.
  J Neurosci 32: 11330-11342 (2012)
• Masuda T*, Tsuda M*#, Yoshinaga R, Tozaki-Saitoh H, Ozato K, Tamura T, Inoue K# (*equal contributors, #corresponding authors)
  IRF8 is a critical transcription factor for transforming microglia into a reactive phenotype.
  Cell Rep 1, 334-340 (2012)　★Selected Paper
• Tsuda M, Beggs S, Salter MW, Inoue K
  Microglia and intractable chronic pain.
  Glia 61: 55-61 (2012)
• Uesugi A, Kataoka A, Tozaki-Saitoh H, Koga Y, Tsuda M, Robaye B, Boeynaems JM, Inoue K
  Involvement of protein kinase D in uridine diphosphate-induced microglial macropinocytosis and phagocytosis.
  Glia 60: 1094-1105 (2012)
• Toyomitsu E, Tsuda M, Yamashita T, Tozaki-Saitoh H, Tanaka Y, Inoue K
  CCL2 promotes P2X4 receptor trafficking to the cell surface of microglia.
  Purinergic Signal 8: 301-310 (2012)
• Tsuda M, Tozaki-Saitoh H, Inoue K
  Purinergic system, microglia and neuropathic pain.
  Curr Opin Pharmacol 12: 74-79 (2012)
• Inoue K, Tsuda M
  Purinergic systems, neuropathic pain and the role of microglia.
  Exp Neurol 234: 293-301 (2012)

2011

• Tsuda M, Tozaki-Saitoh H, Inoue K
  Platelet-activating factor and pain.
  Biol Pharm Bull 34: 1159-1162 (2011)
• Kuboyama K, Tsuda M, Tsutsui M, Toyohara Y, Tozaki-Saitoh H, Shimokawa H, Yanagihara N, Inoue K
  Reduced spinal microglial activation and neuropathic pain after nerve injury in mice lacking all three nitric oxide synthases.
  Mol Pain 7: 50 (2011)
• Tozaki-Saitoh H1, Tsuda M, Inoue K
  Role of purinergic receptors in CNS function and neuroprotection.
  Adv Pharmacol 61: 495-528 (2011)
• Kataoka A, Koga Y, Uesugi A, Tozaki-Saitoh H, Tsuda M, Inoue K
  Involvement of vasodilator-stimulated phosphoprotein in UDP-induced microglial actin aggregation via PKC- and Rho-dependent pathways.
  Purinergic Signal 7: 403-411 (2011)
• Kuboyama K, Harada H, Tozaki-Saitoh H, Tsuda M, Ushijima K, Inoue K
  Astrocytic P2Y1 receptor is involved in the regulation of cytokine/chemokine transcription and cerebral damage in a rat model of cerebral ischemia.
  J Cereb Blood Flow Metab 31: 1930-1941 (2011)
• Biber K*, Tsuda M*, Tozaki-Saitoh H*, Tsukamoto K, Toyomitsu E, Masuda T, Boddeke H, Inoue K (*Equal contributors)
  Neuronal CCL21 up-regulates microglia P2X4 expression and initiates neuropathic pain development.
  EMBO J 30: 1864-1873 (2011)　★Selected Paper
• Tsuda M, Kohro Y, Yano T, Tsujikawa T, Kitano J, Tozaki-Saitoh H, Koyanagi S, Ohdo S, Ji RR, Salter MW, Inoue K
  JAK-STAT3 pathway regulates spinal astrocyte proliferation and neuropathic pain maintenance in rats.
  Brain 134: 1127-1139 (2011)　★Selected Paper

2010

• Kusunose N, Koyanagi S, Hamamura K, Matsunaga N, Yoshida M, Uchida T, Tsuda M, Inoue K, Ohdo S
  Molecular basis for the dosing time-dependency of anti-allodynic effects of gabapentin in a mouse model of neuropathic pain.
  Mol Pain 6: 83 (2010)
• Maeda M, Tsuda M, Tozaki-Saitoh H, Inoue K, Kiyama H
  Nerve injury-activated microglia engulf myelinated axons in a P2Y12 signaling-dependent manner in the dorsal horn.
  Glia 58: 1838-1846 (2010)
• Shiratori M, Tozaki-Saitoh H, Yoshitake M, Tsuda M, Inoue K
  P2X7 receptor activation induces CXCL2 production in microglia through NFAT and PKC/MAPK pathways.
  J Neurochem 114: 810-819 (2010)
• Hasegawa S, Kohro Y, Shiratori M, Ishii S, Shimizu T, Tsuda M, Inoue K
  Role of PAF receptor in proinflammatory cytokine expression in the dorsal root ganglion and tactile allodynia in a rodent model of neuropathic pain.
  PLoS ONE 5: e10467 (2010)
• Toulme E, Tsuda M, Khakh BS, Inoue K
  On the role of ATP-gated P2X receptors in acute, inflammatory and neuropathic pain.
  Translational Pain Research: From Mouse to Man. Boca Raton, FL: CRC Press; Chapter 10 (2010)
• Tsuda M, Tozaki-Saitoh H, Inoue K
  Pain and purinergic signaling.
  Brain Res Rev 63: 222-232 (2010)

2009

• Inoue K, Koizumi S, Kataoka A, Tozaki-Saitoh H, Tsuda M
  P2Y6-evoked microglial phagocytosis.
  Int Rev Neurobiol 85: 159-163 (2009)
• Tsuda M, Kuboyama K, Inoue T, Nagata K, Tozaki-Saitoh H, Inoue K
  Behavioral phenotypes of mice lacking purinergic P2X4 receptors in acute and chronic pain assays.
  Mol Pain 5: 28 (2009)
• Masuda J, Tsuda M, Tozaki-Saitoh H, Inoue K
  Intrathecal delivery of PDGF produces tactile allodynia through its receptors in spinal microglia.
  Mol Pain 5: 23 (2009)
• Tsuda M, Masuda T, Kitano J, Shimoyama H, Tozaki-Saitoh H, Inoue K: IFN-γ receptor signaling mediates spinal microglia activation driving neuropathic pain.
  Proc Natl Acad Sci USA 106: 8032-8037 (2009)　★Selected Paper
• Tsuda M, Toyomitsu E, Kometani M, Tozaki-Saitoh H, Inoue K
  Mechanisms underlying fibronectin-induced upregulation of P2X4R expression in microglia: distinct roles of PI3K-Akt and MEK-ERK signaling pathways.
  J Cell Mol Med 13: 3251-3259 (2009)
• Shinozaki Y, Sumitomo K, Tsuda M, Koizumi S, Inoue K, Torimitsu K
  Direct observation of ATP-induced conformational changes in single P2X4 receptors.
  PLoS Biol 7: e103 (2009)
• Hasegawa S, Kohro Y, Tsuda M, Inoue K
  Activation of cytosolic phospholipase A₂ in dorsal root ganglion neurons by Ca²⁺/calmodulin-dependent protein kinase II after peripheral nerve injury.
  Mol Pain 5: 22 (2009)
• Nagata K, Imai T, Yamashita T, Tozaki-Saitoh H, Tsuda M, Inoue K
  Antidepressants inhibit P2X4 receptor function: a possible involvement in neuropathic pain relief.
  Mol Pain 5: 20 (2009)
• Kataoka A, Tozaki-Saitoh H, Koga Y, Tsuda M, Inoue K
  Activation of P2X7 receptors induces CCL3 production in microglial cells through transcription factor NFAT.
  J Neurochem 108: 115-125 (2009)
• Inoue K, Tsuda M
  Microglia and neuropathic pain.
  Glia 57: 1469-1479 (2009)

2008

• Tozaki-Saitoh H, Tsuda M, Miyata H, Ueda K, Kohsaka S, Inoue K
  P2Y12 receptors in spinal microglia are required for neuropathic pain after peripheral nerve injury.
  J Neurosci 28: 4949-4956 (2008)
• Tsuda M, Toyomitsu E, Komatsu T, Masuda T, Kunifusa E, Nasu-Tada K, Koizumi S, Yamamoto K, Ando J, Inoue K
  Fibronectin/integrin system is involved in P2X4 receptor upregulation in the spinal cord and neuropathic pain after nerve injury.
  Glia 56: 579-585 (2008)
• Tsuda M, Ueno H, Kataoka A, Tozaki-Saitoh H, Inoue K
  Activation of dorsal horn microglia contributes to diabetes-induced tactile allodynia via extracellular signal-regulated protein kinase signaling.
  Glia 56: 378-386 (2008)
• Tsuda M, Tozaki-Saitoh H, Masuda T, Toyomitsu E, Tezuka T, Yamamoto T, Inoue K
  Lyn tyrosine kinase is required for P2X4 receptor upregulation and neuropathic pain after peripheral nerve injury.
  Glia 56: 50-58 (2008)

2007

• Tsuda M, Hasegawa S, Inoue K
  P2X receptors-mediated cytosolic phospholipase A₂ activation in primary afferent sensory neurons contributes to neuropathic pain.
  J Neurochem 103: 1408-1416 (2007)
• Inoue K, Tsuda M, Tozaki-Saitoh H
  Modification of neuropathic pain sensation through microglial ATP receptors.
  Purinergic Signal 3: 311-316 (2007)
• Inoue K, Koizumi S, Tsuda M
  The role of nucleotides in the neuron--glia communication responsible for the brain functions.
  J Neurochem 102: 1447-1458 (2007)
• Tsuda M, Ishii S, Masuda T, Hasegawa S, Nakamura K, Nagata K, Yamashita T, Furue H, Tozaki-Saito H, Yoshimura M, Koizumi S, Shimizu T and Inoue K
  Reduced pain behaviors and ERK activation in primary sensory neurons by peripheral tissue injury in mice lacking platelet-activating factor receptor.
  J Neurochem 102: 1658?1668 (2007)
• Ikeda H, Tsuda M, Inoue K, Murase K
  Long-term potentiation of neuronal excitation by neuron-glia interactions in the rat spinal dorsal horn.
  Eur J Neurosci 25: 1297-1306 (2007)
• Koizumi S, Shigemoto-Mogami Y, Nasu-Tada K, Shinozaki Y, Ohsawa K, Tsuda M, Joshi BV, Jacobson KA, Kohsaka S, Inoue K
  UDP acting at P2Y6 receptors is a mediator of microglial phagocytosis.
  Nature 446: 1091-1095 (2007)　★Selected Paper

2006

• Tozaki-Saitoh H, Koizumi S, Sato Y, Tsuda M, Nagao T, Inoue K
  Retinoic acids increase P2X2 receptor expression through the 5'-flanking region of P2rx2 gene in rat phaeochromocytoma PC-12 cells.
  Mol Pharmacol 70: 319-328 (2006)
• Shiokawa H, Nakatsuka T, Furue H, Tsuda M, Katafuchi T, Inoue K, Yoshimura M
  Direct excitation of deep dorsal horn neurones in the rat spinal cord by the activation of postsynaptic P2X receptors.
  J Physiol 573: 753-763 (2006)
• Nasu-Tada K*, Koizumi S*, Tsuda M*, Kunifusa E, Inoue K (*equal contributors)
  Possible involvement of increase in spinal fibronectin following peripheral nerve injury in upregulation of microglial P2X4, a key molecule for mechanical allodynia.
  Glia 53, 769-775 (2006)

2005

• Coull JA, Beggs S, Boudreau D, Boivin D, Tsuda M, Inoue K, Gravel C, Salter MW, De Koninck Y
  BDNF from microglia causes the shift in neuronal anion gradient underlying neuropathic pain.
  Nature 438: 1017-1021 (2005)　★Selected Paper
• Narita M, Miyatake M, Shibasaki M, Tsuda M, Koizumi S, Narita M, Yajima Y, Inoue K, Suzuki T
  Long-lasting change in brain dynamics induced by methamphetamine: enhancement of protein kinase C-dependent astrocytic response and behavioral sensitization.
  J Neurochem 93: 1383-1392 (2005)
• Tsuda M, Inoue K, Salter MW
  Neuropathic pain and spinal microglia: a big problem from molecules in "small" glia.
  Trends Neurosci 28: 101-107 (2005)
• Inoue K, Tsuda M, Koizumi S
  ATP receptors in pain sensation: Involvement of spinal microglia and P2X(4) receptors.
  Purinergic Signal 1: 95-100 (2005)

2004

• Inoue K, Tsuda M, Koizumi S
  ATP- and adenosine-mediated signaling in the central nervous system: chronic pain and microglia: involvement of the ATP receptor P2X4.
  J Pharmacol Sci 94: 112-114 (2004)
• Koizumi S, Fujishita K, Inoue K, Shigemoto-Mogami Y, Tsuda M, Inoue K
  Ca²⁺ waves in keratinocytes are transmitted to sensory neurons: the involvement of extracellular ATP and P2Y2 receptor activation.
  Biochem J 380: 329-338 (2004)
• Tsuda M, Mizokoshi A, Shigemoto-Mogami Y, Koizumi S, Inoue K:
  Activation of p38 mitogen-activated protein kinase in spinal hyperactive microglia contributes to pain hypersensitivity following peripheral nerve injury.
  Glia 45: 89-95 (2004)

2003

• Inoue K, Koizumi S, Tsuda M, Shigemoto-Mogami Y
  Signaling of ATP receptors in glia-neuron interaction and pain.
  Life Sci 74: 189-197 (2003)
• Koizumi S, Fujishita K, Tsuda M, Shigemoto-Mogami Y, Inoue K
  Dynamic inhibition of excitatory synaptic transmission by astrocyte-derived ATP in hippocampal cultures.
  Proc Natl Acad Sci USA 100: 11023-11028 (2003 )
• Tsuda M, Shigemoto-Mogami Y, Koizumi S, Mizokoshi A, Kohsaka S, Salter MW, Inoue K
  P2X4 receptors induced in spinal microglia gate tactile allodynia after nerve injury.
  Nature 424: 778-783 (2003)　★Selected Paper

2002～

• Tsuda M, Shigemoto-Mogami Y, Ueno S, Koizumi S, Ueda H, Iwanaga T, Inoue K
  Downregulation of P2X3 receptor-dependent sensory functions in A/J inbred mouse strain.
  Eur J Neurosci 15: 1444-1450 (2002)
• Shigemoto-Mogami Y, Koizumi S, Tsuda M, Ohsawa K, Kohsaka S, Inoue K
  Mechanisms underlying extracellular ATP-evoked interleukin-6 release in mouse microglial cell line, MG-5.
  J Neurochem 78: 1339-1349 (2001)
• Tsuda M, Koizumi S, Inoue K
  Role of endogenous ATP at the incision area in a rat model of postoperative pain.
  Neuroreport 12: 1701-1704 (2001)
• Tsuda M, Koizumi S, Kita A, Shigemoto Y, Ueno S, Inoue K
  Mechanical allodynia caused by intraplantar injection of P2X receptor agonist in rats: involvement of heteromeric P2X2/3 receptor signaling in capsaicin-insensitive primary afferent neurons.
  J Neurosci 20: RC90 (2000)
• Tsuda M, Ueno S, Inoue K
  In vivo pathway of thermal hyperalgesia by intrathecal administration of alpha,beta-methylene ATP in mouse spinal cord: involvement of the glutamate-NMDA receptor system.
  Br J Pharmacol 127: 449-456 (1999)
• Tsuda M, Ueno S, Inoue K
  Evidence for the involvement of spinal endogenous ATP and P2X receptors in nociceptive responses caused by formalin and capsaicin in mice.
  Br J Pharmacol 128: 1497-1504 (1999)
• Ueno S, Tsuda M, Iwanaga T, Inoue K
  Cell type-specific ATP-activated responses in rat dorsal root ganglion neurons.
  Br J Pharmacol 126: 429-436 (1999)